GLYCEROL MONOSTEARAT 40 - 55

Glyceroli monostearas 40-45

 

Glycerol monostearat 40 - 55 la hon hp cac monoacylglycerol, chu yeu la monostearoylglycerol, cung vi di va triacylglycerol vi ham lng khac nhau. Che pham phai cha t 40,0% en 55,0% monoacylglycerol, 30,0% en 45,0% diacylglycerol va en 5 - 15% triacylglycerol, thu c bang cach phan giai glycerol tng phan dau thc vat cha chu yeu triacylglycerol cua acid palmitic hoac acid stearic, hay bang cach ester hoa glycerol vi acid stearic 50 (loai I), acid stearic 70 (loai II) hoac acid stearic 95 (loai III). Cac acid beo co the co nguon goc t ong vat hoac thc vat.

Tnh chat

Khoi sap cng hoac bot, vay nhn, mau trang hoac gan nh trang. Thc te khong tan trong nc, tan trong ethanol 96% 60 oC.

nh tnh

A. Nhiet o nong chay

54 64 oC (Phu luc 6.7)

Cho che pham vao ong mao quan va at trong bnh kn trong vong 24 gi.

B. Phng phap sac ky lp mong (Phu luc 5.4)

Ban mong: Silica gel (TT).

Dung moi khai trien: Hexan - ether (30 : 70)

Dung dch th: Hoa tan 1,0 g che pham trong methylen clorid (TT) bang cach un nhe va pha loang en 20 ml vi cung dung moi.

Dung dch oi chieu: Hoa tan 1,0 g glycerol monostearat 40 - 55 chuan (C) trong methylen clorid (TT) bang cach un nhe va pha loang en 20 ml vi cung dung moi.

Cach tien hanh: Cham rieng biet len ban mong 10 l moi dung dch tren. Trien khai sac ky en khi dung moi i c 15 cm. Lay ban mong ra va e kho ngoai khong kh. Phun dung dch rhodamin B 0,01% trong ethanol 96% (TT), quan sat di anh sang t ngoai 365 nm. Cac vet thu c tren sac ky o cua dung dch th phai co v tr giong vi vet tren sac ky o cua dung dch oi chieu.

C. ap ng phep th thanh phan acid beo tuy theo loai acid beo c qui nh tren nhan.

D. ap ng cac gii han nh lng (ham lng monoacylglycerol).

Ch so acid

Khong c qua 3,0 (Phu luc 7.2).

Dung 1,0 g che pham pha trong hon hp ong the tch ethanol 96% (TT) va toluen (TT), un nong nhe.

Ch so iod

Khong c qua 3,0 (Phu luc 7.5).

Ch so xa phong hoa

158 -177 (Phu luc 7.7).

Dung 2,0 g che pham. un nong khi tien hanh nh lng.

Glycerol t do

Khong c qua 6,0%, tien hanh nh phan nh lng.

Thanh phan acid beo

Thanh phan acid beo cua che pham phai at yeu cau theo bang (Phu luc 10, phng phap C)

 

 

Acid beo dung e ester hoa

Thanh phan acid beo

Glycerol monostearat 40 - 55 (loai I)

Acid stearic 50

Acid stearic: 40,0 - 60,0%

Tong lng acid palmitic va acid stearic: khong thap hn 90,0%.

Glycerol monostearat 40 - 55 (loai II)

Acid stearic 70

Acid stearic: 60,0 - 80,0%

Tong lng acid palmitic va acid stearic: khong thap hn 90,0%.

Glycerol monostearat 40 - 55 (loai III)

Acid stearic 95

Acid stearic: 90,0 - 99,0%

Tong lng acid palmitic va acid stearic: khong thap hn 96,0%.

Nickel

Khong c qua 1 phan trieu (Phu luc 9.4.11).

Nc

Khong c qua 1,0% (Phu luc 10.3)

Dung 1,0 g che pham va pyridin (TT) lam dung moi, un nong nhe.

Tro toan phan

Khong c qua 0,1% (Phu luc 9.8).

Dung 1,0 g che pham.

nh lng

Xac nh ham lng glycerol t do va ham lng di va triacylglycerol bang phng phap sac ky ray phan t (Phu luc 5.5).

ieu kien sac ky

Cot: Cot tham thau gel dai 0,6 m, ng knh trong 7 mm c nhoi styren- divinylbenzen copolymer (TT) (ng knh tieu phan 5 m, kch thc lo 10 nm).

Pha ong: tetrahydrofuran (TT).

Toc o dong 1 ml/phut.

Detector: khuc xa ke vi sai.

The tch tiem : 40 l.

Dung dch th

Can 0,2 g (chnh xac en 0,1 mg) che pham (m) cho vao bnh 15 ml. Them 5 ml tetrahydrofuran (TT), lac manh e hoa tan. Can lai bnh, tnh tong khoi lng dung moi va che pham (M)

Dung dch oi chieu

Can lan lt 2,5 0,1 mg, 5 0,1 mg, 10 0,1 mg, 20 0,1 mg glycerol (TT) cho vao 4 bnh 15 ml. Them vao moi bnh 5 ml tetrahydrofuran (TT), lac manh e hoa tan. Can lai cac bnh va tnh nong o glycerol (mg/g) cho moi dung dch oi chieu.

Cach tien hanh

Tiem moi dung dch. Trong ieu kien mo ta tren, sac ky o thu c co thi gian lu tng oi so vi thi gian lu cua glycerol la khoang 0,86 oi vi monoacylglycerol, khoang 0,81 oi vi diacylglycerol va 0,77 oi vi tricylglycerol. T ng cong chuan thu c cua cac dung dch oi chieu, xac nh nong o C (mg/g) cua glycerol trong dung dch th.

Ham lng % glycerol t do trong che pham c tnh bang cong thc:

Ham lng (%) cua mono, di va triacylglycerol c xac nh bang phng phap chuan hoa.

Nhan

Phai qui nh loai glycerol monostearat 40 - 55.

Bao quan

Bao b kn, tranh anh sang

Loi thuc

Ta dc.