CLOROQUIN PHOSPHAT

Cloroquini phosphas

Cloroquin diphosphat, nivaquin phosphat, aralen

 

 

 

va ong phan oi quang

 

 

 

C18H26ClN3. 2H3PO4 P.t.l: 515,9

Cloroquin phosphat la (RS) -4- (7-cloro-4-quinolylamino) pentyldiethylamin diphosphat, phai cha t 98,5 en 101,0% C18H26ClN3. 2H3PO4, tnh theo che pham a lam kho.

Tnh chat

Bot ket tinh trang hoac gan nh trang, khong mui, v ang, de bien mau khi e ngoai anh sang, de hut am.

De tan trong nc, rat kho tan trong cloroform, ethanol 96%, ether va methanol. Ton tai 2 dang, mot dang chay khoang 195 oC va dang khac chay khoang 218 oC.

nh tnh

Co the chon mot trong hai nhom nh tnh sau:

Nhom I: A, D.

Nhom II: B, C, D.

A. Hoa tan 0,1 g che pham trong 10 ml nc, them 2 ml dung dch natri hydroxyd 2 M (TT), chiet 2 lan, moi lan vi 20 ml methylen clorid (TT). Ra dch methylen clorid vi nc, lam khan bang natri sulfat khan (TT) lam bay hi en kho va hoa can trong 2 ml methylen clorid (TT). Pho hong ngoai (Phu luc 4.2) cua dung dch nay phai giong pho hong ngoai oi chieu cua dung dch thu c t 80 mg cloroquin sulfat chuan (C) vi cach chuan b tng t.

B. Pho hap thu t ngoai (Phu luc 4.1) cua dung dch che pham 0,001% trong nc bc song t 210 en 370 nm cho cac cc ai hap thu lan lt 220, 235, 256, 329 va 342 nm. A (1%, 1 cm) tng ng lan lt la 600 en 660; 350 en 390; 300 en 330; 325 en 355 va 360 en 390.

C. Hoa tan 25 mg che pham trong 20 ml nc, them 5 ml dung dch acid picric (TT) se xuat hien tua vang. Loc va ra tua lan lt vi nc, ethanol 96% (TT) va methylen clorid (TT). Tua nay co iem chay t 206 en 209 oC (Phu luc 6.7).

D. Hoa tan 0,1 g che pham trong 10 ml nc, them 2 ml dung dch natri hydroxyd 2 M (TT), chiet 2 lan, moi lan vi 10 ml methylen clorid (TT). Lp nc sau khi c acid hoa bang acid nitric (TT) cho phan ng cua phosphat (Phu luc 8.1).

o trong va mau sac cua dung dch.

Dung dch S phai trong (Phu luc 9.2) va mau khong c am hn mau cua mau mau VN5 hay VL5 (Phu luc 9.3, phng phap 2).

pH

Dung dch S: Hoa tan 2,50 g che pham trong nc khong co carbon dioxyd (TT), pha loang thanh 25 ml vi cung dung moi

pH cua dung dch S phai t 3,8 en 4,3 (Phu luc 6.2).

Tap chat lien quan

Xac nh bang phng phap sac ky lp mong (Phu luc 5.4).

Ban mong: Silicagel GF254 (TT).

Dung moi khai trien: Cloroform - cyclohexan - diethylamin (50 : 40 : 10).

Dung dch th: 5,0% che pham trong nc.

Dung dch oi chieu (1): 0,050% che pham trong nc.

Dung dch oi chieu (2): 0,025% che pham trong nc.

Cach tien hanh: Cham rieng biet len ban mong 2 ml moi dung dch tren. Trien khai sac ky en khi dung moi i c khoang 12 cm. Sau khi trien khai, lay ban mong ra e kho ngoai khong kh va quan sat di anh sang t ngoai bc song 254 nm. Bat c vet phu nao tren sac ky o thu c t dung dch th cung khong c am mau hn vet tren sac ky o thu c t dung dch oi chieu (1) va ch c co mot vet nh vay am mau hn vet tren sac ky o thu c t dung dch oi chieu (2).

Kim loai nang

Khong c qua 20 phan trieu (Phu luc 9.4.8).

Hoa tan 2,0 g che pham trong 10 ml nc, them 5 ml dung dch amoniac 13,5 M (TT) va lac vi 40 ml methylen clorid (TT). Loc lay lp nc, trung hoa dch loc bang acid acetic bang (TT), un nong tren cach thuy e loai het methylen clorid, lam lanh va pha loang vi nc va u 20 ml. Lay 12 ml dung dch nay tien hanh theo phng phap 1. Dung dung dch ch mau 2 phan trieu (C) e chuan b mau oi chieu.

Mat khoi lng do lam kho

Khong c qua 2,0% (Phu luc 9.6).

(1,000 g; 100 -105 oC)

nh lng

Can 0,200 g che pham, hoa tan trong 50 ml acid acetic khan (TT), them 2 giot dung dch tm tinh the (TT). nh lng bang dung dch acid percloric 0,1 N (C) cho ti khi dung dch chuyen sang mau xanh luc hoac co the xac nh iem ket thuc bang phng phap chuan o o ien the (Phu luc 10.2).

1 ml dung dch acid percloric 0,1 N (C) tng ng vi 25,79 mg C18H26ClN3. 2H3PO4.

Bao quan

Trong lo kn, tranh anh sang.

Che pham

Thuoc tiem, vien bao ng, vien nen.

Tac dung va cong dung

Tr sot ret.